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AM真菌和根瘤菌互作对苜蓿根际土壤真菌群落结构的影响及功能预测 S2

杨盼 翟亚萍 赵祥 王绍明 刘红玲 张霞

杨盼,翟亚萍,赵祥,王绍明,刘红玲,张霞. AM真菌和根瘤菌互作对苜蓿根际土壤真菌群落结构的影响及功能预测. 草业科学, 2020, 37(9): 1669-1680 doi: 10.11829/j.issn.1001-0629.2019-0622
引用本文: 杨盼,翟亚萍,赵祥,王绍明,刘红玲,张霞. AM真菌和根瘤菌互作对苜蓿根际土壤真菌群落结构的影响及功能预测. 草业科学, 2020, 37(9): 1669-1680 doi: 10.11829/j.issn.1001-0629.2019-0622
YANG P, ZHAI Y P, ZHAO X, WANG S M, LIU H L, ZHANG X. Effect of arbuscular mycorrhizal fungi and rhizobium inoculation on soil fungal community structure and function in the rhizosphere of Medicago sativa. Pratacultural Science, 2020, 37(9): 1669-1680 doi: 10.11829/j.issn.1001-0629.2019-0622
Citation: YANG P, ZHAI Y P, ZHAO X, WANG S M, LIU H L, ZHANG X. Effect of arbuscular mycorrhizal fungi and rhizobium inoculation on soil fungal community structure and function in the rhizosphere of Medicago sativa. Pratacultural Science, 2020, 37(9): 1669-1680 doi: 10.11829/j.issn.1001-0629.2019-0622

AM真菌和根瘤菌互作对苜蓿根际土壤真菌群落结构的影响及功能预测 S2

doi: 10.11829/j.issn.1001-0629.2019-0622
基金项目: 国家自然科学基金(31560656、31871568)
详细信息
    作者简介:

    杨盼(1994-),女,甘肃酒泉人,在读硕士生,研究方向为植物遗传多样性。E-mail: yp526032479@163.com

    通讯作者:

    张霞(1964-),女,新疆奎屯人,教授,学士,研究方向为植物遗传多样性。E-mail: xiazh@shzu.edu.cn

Effect of arbuscular mycorrhizal fungi and rhizobium inoculation on soil fungal community structure and function in the rhizosphere of Medicago sativa

More Information
  • 摘要:

    在我国,优良豆科牧草紫花苜蓿(Medicago sativa)一直处于供不应求的状态,接种AM (arbuscular mycorrhizae)真菌和根瘤菌可以促进土壤氮、磷循环以及提高植株抗逆性,从而提高苜蓿产量。为探究接种AM真菌和根瘤菌对苜蓿根际真菌群落结构和功能的影响,采集苜蓿6个不同处理组(不接种、单接种摩西球囊霉、单接种根内球囊霉、单接种根瘤菌、双接种根内球囊霉和根瘤菌、双接种摩西球囊霉和根瘤菌)根际和非根际土壤样品,基于真菌ITS区高通量测序技术,分析比较不同接种处理组苜蓿根际、非根际土壤中真菌多样性和群落分布的规律,并采用FUNGuild软件对不同处理组间菌群功能进行预测。结果表明:6个不同处理组中,在门水平上土壤真菌群落主要由子囊菌门、担子菌门构成,且以子囊菌门为主(19.29%~61.18%)。在属水平上发现与不接种相比,接种AM真菌和根瘤菌显著提高了有益菌的丰度,如单接种摩西球囊霉GMR处理组中优势属为支顶孢属(Acremonium)、单接种根瘤菌KR处理组中优势属为葡萄穗霉属(Stachybotrys)和毛束霉属(Trichurus),双接种根内球囊霉和根瘤菌GIKR处理组中优势属为木霉属(Trichoderma),双接种摩西球囊霉和根瘤菌GMKR处理组中优势属为曲霉菌属(Aspergillus)。与不接种相比,接种AM真菌和根瘤菌提高了真菌群落的操作分类单元数(OTUs)、Shannon-Wiener指数、菌种丰富度Chao1指数。不同接种处理间主要以腐生营养型为主,接种AM真菌和根瘤菌均会增加共生营养型的相对丰度,降低病原营养型的相对丰度。综上所述,接种AM真菌和根瘤菌后,有利于土壤氮磷的循环,增加了土壤真菌群落的丰富度与多样性,在致病菌存在的同时增加了生防菌,以及与固氮、溶磷有关菌的丰度,增加了共生营养型的相对丰度,降低了病原营养型的相对丰度,故接种 根瘤菌丰富了真菌群落组成,优化了原有的微生物生态环境,从而有利于苜蓿植株品质及产量的提高。

  • 图  1  不同接种处理下苜蓿土壤根际真菌门水平的相对丰度弦图

    a, Ascomycota 子囊菌门; b, Mortierellomycota 被孢霉门; c, Basidiomycota 担子菌门; d, Mucoromycota 毛霉菌门; e, Glomeromycota 球囊菌门; f, Aphelidiomycota; g, Chytridiomycota 壶菌门; h, Rozellomycota 罗兹菌门; i, Calcarisporiellomycota; j, Blastocladiomycota 芽枝霉门; k, others其他.

    Figure  1.  Relative abundance chord diagram of rhizosphere fungal division levels of alfalfa under different inoculation treatments

    图  2  不同接种处理下苜蓿土壤真菌群落基于属水平上的聚类分析

    Figure  2.  Cluster analysis of alfalfa soil fungal community based on genus level under different inoculation treatments

    图  3  不同接种处理苜蓿土壤真菌功能多样性热图

    Figure  3.  Heat map of fungal functional diversity in alfalfa soil under different inoculation treatments

    表  1  苜蓿各试验组样本土壤的理化因子

    Table  1.   Value for soil physical and chemical factors of each testing group sample

    样品分组
    Group name
    pH 有机质含量
    OM content/
    (g·kg−1)
    全氮含量
    TN content/
    (g·kg−1)
    全磷含量
    TP content/
    (g·kg−1)
    有效磷含量
    AP content/
    (mg·kg−1)
    碱解氮含量
    AN content/
    (g·kg−1)
    过氧化氢酶活性
    CAT activity/
    (U·mL−1)
    中性磷酸酶活性NP activity/
    (U·L−1)
    CKTR 8.19 ± 0.00abcd 29.10 ± 0.39de 0.73 ± 0.14a 1.47 ± 0.06a 31.85 ± 1.35ab 57.93 ± 2.33cd 0.38 ± 0.06ab 0.45 ± 0.02f
    CKTB 8.12 ± 0.01d 47.35 ± 0.40a 0.67 ± 0.03bc 1.34 ± 0.02bc 23.80 ± 2.57cd 63.67 ± 3.24bc 0.34 ± 0.04cd 0.25 ± 0.05i
    GIR 8.28 ± 0.01a 22.10 ± 0.25g 0.57 ± 0.05de 1.15 ± 0.09de 18.33 ± 3.66e 59.00 ± 3.16cd 0.33 ± 0.03cd 0.47 ± 0.01e
    GIB 8.22 ± 0.01abc 20.93 ± 0.45g 0.51 ± 0.32f 1.03 ± 0.02f 27.82 ± 5.89bc 58.83 ± 2.56cd 0.22 ± 0.01f 0.24 ± 0.03g
    GMR 8.22 ± 0.00abc 32.73 ± 0.36bc 0.52 ± 0.24f 1.05 ± 0.08f 22.64 ± 3.67de 58.50 ± 2.79cd 0.37 ± 0.02b 0.55 ± 0.01c
    GMB 8.26 ± 0.00ab 21.76 ± 0.55g 0.56 ± 0.09ef 1.12 ± 0.05ef 23.65 ± 4.44cd 73.50 ± 3.87a 0.27 ± 0.02e 0.22 ± 0.02k
    KR 8.16 ± 0.01bcd 35.96 ± 0.53b 0.59 ± 0.06de 1.19 ± 0.03de 32.00 ± 5.35ab 66.00 ± 5.21b 0.40 ± 0.03a 0.58 ± 0.03b
    KB 8.17 ± 0.00bcd 25.94 ± 0.46ef 0.71 ± 0.01ab 1.41 ± 0.04ab 31.71 ± 6.65ab 56.00 ± 4.23d 0.32 ± 0.01d 0.40 ± 0.01g
    GIKR 8.14 ± 0.00cd 26.77 ± 0.37ef 0.70 ± 0.06ab 1.39 ± 0.08ab 31.13 ± 4.34ab 58.17 ± 4.67cd 0.34 ± 0.04cd 0.60 ± 0.02ab
    GIKB 8.17 ± 0.01bcd 23.38 ± 0.42fg 0.72 ± 0.01a 1.44 ± 0.07a 35.74 ± 5.42a 65.33 ± 5.33b 0.34 ± 0.01c 0.31 ± 0.04h
    GMKR 8.25 ± 0.01ab 31.76 ± 0.40cd 0.66 ± 0.06bc 1.32 ± 0.09bc 21.21 ± 6.67de 68.33 ± 3.47ab 0.34 ± 0.03cd 0.60 ± 0.01a
    GMKB 8.23 ± 0.00abc 25.70 ± 0.44ef 0.62 ± 0.01cd 1.24 ± 0.07cd 21.35 ± 3.84de 68.30 ± 3.18bc 0.34 ± 0.01cd 0.50 ± 0.03d
     CKTR:不接种处理组根际土壤;CKTB:不接种处理组非根际土壤;GIR:单接种根内球囊霉处理组根际土壤;GIB:单接种根内球囊霉处理组非根际土壤;GMR:单接种摩西球囊霉处理组根际土壤;GMB:单接种摩西球囊霉处理组非根际土壤;KR:单接种根瘤菌处理组根际土壤;KB:单接种根瘤菌处理组非根际土壤;GIKR:双接种根内球囊霉和根瘤菌处理组根际土壤;GIKB:双接种根内球囊霉和根瘤菌处理组非根际土壤;GMKR:双接种摩西球囊霉和根瘤菌处理组根际土壤;GMKB:双接种摩西球囊霉和根瘤菌处理组非根际土壤;下同。OM:有机质;TN:全氮;TP:全磷;AP:有效磷;AN:碱解氮;CAT:过氧化氢酶;NP:中性磷酸酶;同列不同小写字母表示不同样品分组之间差异显著(P < 0.05);下同。

     CKTR: Rhizosphere soil of non-inoculation treatment group; CKTB: Non-rhizosphere soil in non-inoculation treatment group; GIR: Rhizosphere soil of only inoculation withGlomus intraradices treatment group; GIB: Non-rhizosphere soil of only inoculation withGlomus intraradices treatment group; GMR: Rhizosphere soil of only inoculationGlomus mosseae treatment group; GMB: Non-rhizosphere soil of only inoculation withGlomus mosseaetreatment group; KR: Rhizosphere soil of only inoculation Sinorhizobium melilotitreatment group; KB: Non-rhizosphere soil of only inoculation withSinorhizobium melilotitreatment group; GIKR: Rhizosphere soil of double inoculation with Glomus intraradicesand Sinorhizobium meliloti treatment group; GIKB: Non-rhizosphere soil of double inoculation with Glomus intraradicesand Sinorhizobium meliloti treatment group; GMKR: Rhizosphere soil of double inoculation with Glomus mosseaeand Sinorhizobium meliloti treatment group; GMKB: Non-rhizosphere soil of double inoculation with Glomus mosseaeand Sinorhizobium melilotitreatment group; this is applicable for the following figures and tables as well. OM: organic matter; TN: total nitrogen; TP: total phosphorus; AP: available phosphorus; AN: alkali nitrogen; CAT: Catalase; NP: Neutral phosphatase. Different lowercase letters within the same column indicate significant differences between different sample groups at the 0.05 level; this is applicable for the following figures as well.
    下载: 导出CSV

    表  2  不同接种处理组苜蓿根际、非根际土壤真菌丰度和多样性指数

    Table  2.   Fungal abundance and diversity index of alfalfa rhizosphere and non-rhizosphere soil

    样品分组
    Group name
    OTUs数
    OTUs
    Shannon-Wiener指数
    Shannon-Wiener index
    Chao1 指数
    Chao1 index
    覆盖率
    Coverage/%
    CKTR 908.01 ± 55.91c 5.76 ± 0.09a 1101.42 ± 16.78c 0.993 0 ± 0.000 1a
    CKTB 991.11 ± 53.21c 6.51 ± 0.33a 1212.90 ± 16.45bc 0.991 0 ± 0.000 3a
    GIR 1101.08 ± 78.32bc 6.31 ± 0.12a 1376.79 ± 12.34bc 0.991 0 ± 0.000 1a
    GIB 1041.35 ± 45.31c 5.95 ± 0.16a 1368.43 ± 14.23bc 0.990 0 ± 0.000 1a
    GMR 1077.24 ± 65.16c 6.35 ± 0.19a 1361.18 ± 15.32bc 0.990 0 ± 0.000 2a
    GMB 2633.17 ± 123.51a 7.27 ± 0.35a 4967.61 ± 33.62a 0.996 0 ± 0.000 1a
    KR 2083.26 ± 136.80ab 7.57 ± 0.21a 3272.07 ± 16.34ab 0.982 0 ± 0.000 3a
    KB 1025.35 ± 64.78c 5.76 ± 0.16a 1291.18 ± 18.95bc 0.991 0 ± 0.000 1a
    GIKR 1254.26 ± 63.21bc 6.70 ± 0.31a 1613.12 ± 23.16bc 0.989 0 ± 0.000 3a
    GIKB 1090.32 ± 79.32bc 6.67 ± 0.15a 1420.70 ± 25.14bc 0.990 0 ± 0.000 1a
    GMKB 1070.17 ± 78.32bc 6.43 ± 0.18a 1346.61 ± 31.21bc 0.990 0 ± 0.000 1a
    GMKR 1099.11 ± 64.21bc 6.31 ± 0.32a 1409.56 ± 22.78bc 0.990 0 ± 0.000 2a
    下载: 导出CSV
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  • 收稿日期:  2019-12-24
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