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重复经颅磁刺激显著改善小鼠老化过程中认知损伤及提高神经元兴奋性

朱海军 丁冲 李洋 徐桂芝

朱海军, 丁冲, 李洋, 徐桂芝. 重复经颅磁刺激显著改善小鼠老化过程中认知损伤及提高神经元兴奋性[J]. 仁和测试, 2020, 37(3): 380-388. doi: 10.7507/1001-5515.201905072
引用本文: 朱海军, 丁冲, 李洋, 徐桂芝. 重复经颅磁刺激显著改善小鼠老化过程中认知损伤及提高神经元兴奋性[J]. 仁和测试, 2020, 37(3): 380-388. doi: 10.7507/1001-5515.201905072
Haijun ZHU, Chong DING, Yang LI, Guizhi XU. Repetitive transcranial magnetic stimulation significantly improves cognitive impairment and neuronal excitability during aging in mice[J]. Rhhz Test, 2020, 37(3): 380-388. doi: 10.7507/1001-5515.201905072
Citation: Haijun ZHU, Chong DING, Yang LI, Guizhi XU. Repetitive transcranial magnetic stimulation significantly improves cognitive impairment and neuronal excitability during aging in mice[J]. Rhhz Test, 2020, 37(3): 380-388. doi: 10.7507/1001-5515.201905072

重复经颅磁刺激显著改善小鼠老化过程中认知损伤及提高神经元兴奋性

doi: 10.7507/1001-5515.201905072
基金项目: 国家自然科学基金重点项目(51737003);国家自然科学基金面上项目(51677053);国家自然科学基金青年基金(51507046);河北省博士后科研项目择优资助(B2015003013)
详细信息
    通讯作者:

    丁冲,Email:dingchong@hebut.edu.cn

    徐桂芝,Email:gzxu@hebut.edu.cn

Repetitive transcranial magnetic stimulation significantly improves cognitive impairment and neuronal excitability during aging in mice

Funds: The National Natural Science Foundation of China
More Information
  • 摘要: 重复经颅磁刺激是一种无创的脑刺激技术,作为一种治疗性神经康复手段备受关注。已有研究表明,高频重复经颅磁刺激可以提高动物在行为测试中的认知能力和神经元兴奋性。本文旨在研究小鼠自然老化过程中,高频重复经颅磁刺激对其认知能力和神经兴奋性的影响。实验采用青年小鼠、成年小鼠、老年小鼠各 12 只,且每个年龄段小鼠被随机分成刺激组和对照组。刺激组小鼠接受连续 15 天的高频重复经颅磁刺激,对照组接受连续 15 天的伪刺激。刺激结束之后,进行新物体识别与跳台测试,用以检查小鼠的学习记忆能力。行为学测试结束之后,进行全细胞脑片膜片钳实验,用以记录并分析海马齿状回颗粒神经元的静息膜电位、动作电位及其相关电特性指标。数据分析表明,小鼠认知能力与神经兴奋性随着老化而显著衰退,高频重复经颅磁刺激能显著改善认知损伤并缓解神经电特性指标的衰退。通过改变海马齿状回颗粒神经元电生理特性以及提高神经元兴奋性,可能是重复经颅磁刺激缓解认知损伤、提高认知能力的机制之一。
  • 图  1  实验设计流程图

    Figure  1.  Experimental design

    图  2  新物体识别实验流程图

    Figure  2.  Experimental protocol of novel object recognition test

    图  3  跳台实验流程图

    Figure  3.  Experimental protocol of step-down test

    图  4  动作电位相关指标示意图

    Figure  4.  Schematic diagram of related indexes of action potential

    图  5  新物体识别认知指数

    a. 测试Ⅰ期认知指数;b.测试Ⅱ期认知指数。**P < 0.01,***P < 0.001

    Figure  5.  Cognitive index of novel object recognition test

    a. cognitive index of test Ⅰ phase; b. cognitive index of test Ⅱ phase. **P < 0.01, ***P < 0.001

    图  6  跳台实验数据分析

    a. 潜伏期;b. 犯错误次数;c. 跳台停留时间百分比;d. 被动逃避潜伏期。**P < 0.01,***P < 0.001

    Figure  6.  Data analysis of step-down test

    a. step-down latency; b. number of mistakes; c. percentage of residence time on the platform; d. passive escape latency. **P < 0.01, ***P < 0.001

    图  7  膜电位及动作电位相关电特性数据分析

    a. 静息膜电位;b. 动作电位发放个数;c. 后超极化电位;d. 动作电位峰值;e. 动作电位达峰时间;f. 动作电位上升支平均斜率;g. 动作电位下降支平均斜率。*P < 0.05,**P < 0.01,***P < 0.001

    Figure  7.  Data analysis of membrane potential and related electrical characteristics of action potential (AP)

    a. resting membrane potential; b. the number of APs released; c. after-hyperpolarizing potential; d. AP peak amplitude; e. time to AP peak amplitude; f. average rise slope of AP; g. average down slope of AP. *P < 0.05, **P < 0.01, ***P < 0.001

    表  1  切片液、人工脑脊液与电极内液离子成分

    Table  1.   The ionic composition of cutting solution, artificial cerebrospinal fluid and internal solutions

    名称 成分/(mmol·L−1) pH 渗透压/mOsm
    切片液 KCl 2.5, CaCl2 1, MgCl2 6, NaH2PO4·2H2O 1.625, NaHCO3 26, glucose 11, sucrose 220 7.4
    人工脑脊液 NaCl 124, KCl 3, CaCl2 2, MgCl2 2, NaH2PO4·2H2O 1.625, NaHCO3 26, glucose 11, HEPES 5 7.4 310~320
    电极内液 K-gluconate 125, NaCl 15, MgCl2 2, CaCl2 1, EGTA 11, HEPES 10, Na-ATP 3, Na-GTP 0.3 7.2~7.3 285~290
    下载: 导出CSV
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出版历程
  • 收稿日期:  2019-05-27
  • 修回日期:  2020-03-20
  • 刊出日期:  2020-03-17

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